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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is evaluating a new rapid diagnostic test for influenza viruses. The test has a high sensitivity but a lower specificity compared to RT-PCR. In a setting with a low prevalence of influenza, the use of this rapid test is MOST likely to result in a high number of:

A) False positive results
B) False negative results
C) True negative results
D) True positive results

2. A 6-year-old child presents with a pharyngitis characterized by a grayish-white pseudomembrane covering the tonsils and posterior pharynx. Gram stain of the membrane reveals Gram-positive bacilli. Culture on Loeffler's medium shows growth of metachromatic granules. The MOST significant virulence factor associated with this organism's pathogenicity is:

A) Protein A
B) Hyaluronidase
C) Streptolysin O
D) Diphtheria toxin

3. A clinical microbiology laboratory is evaluating a new multiplex PCR assay for the detection of respiratory viruses. To assess the assay's specificity, they test a panel of samples known to contain various bacterial pathogens. A positive result is obtained for rhinovirus in a sample containing only Streptococcus pneumoniae.
This indicates:

A) A mixed infection in the original patient sample.
B) Poor analytical sensitivity of the PCR assay for rhinovirus.
C) Degradation of the rhinovirus target sequence during storage.
D) Cross-reactivity of the PCR primers with Streptococcus pneumoniae DNA.

4. A clinical trial is evaluating the efficacy of a new antifungal agent against invasive aspergillosis. Serum galactomannan levels are being used as a biomarker to monitor the infection. A decrease in galactomannan levels during treatment would indicate:

A) A false-positive result in the galactomannan assay.
B) Successful clearance or reduction of the Aspergillus burden.
C) Development of resistance to the antifungal agent.
D) Concurrent infection with a non-Aspergillus mold.

5. A patient with a history of chronic lung disease develops a severe pneumonia. Bronchoalveolar lavage fluid reveals the presence of numerous cysts containing multiple nuclei, which stain positively with silver stains but poorly with Gram stain. The patient's CD4+ T-cell count is within the normal range. The MOST likely causative organism and the preferred treatment are:

A) Histoplasma capsulatum; amphotericin B followed by itraconazole.
B) Pneumocystis jirovecii; trimethoprim-sulfamethoxazole.
C) Blastomyces dermatitidis; itraconazole.
D) Paragonimus westermani; praziquantel.

Solutions: